Organization
School of Medicine (Life Sciences and Social Medicine) Department of Cellular and Integrative Physiology Postdoctoral Researcher
Title
Postdoctoral Researcher
Contact information
SHIMURA Kiyohito
Degree
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Ph. D. (Pharmaceutical Science) ( 1985.3 Hokkaido University )
Research Interests
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capillary electrophoresis, isoelectric focusing, bioaffinity, molecular interaction, immunoassay
Research Areas
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Nanotechnology/Materials / Analytical chemistry / Bioanalytical Chemistry
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Others / Others / Capillary Electrophoresis
Education
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Hokkaido University Graduate School, Division of Pharmaceutical Sciences Pharmaceutical Biochemistry
- 1979.3
Country： Japan
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Graduate School of Hokkaido University Faculty of Pharmaceutical Sciences Biological Chemistry
- 1979.3
Country： Japan
Research History
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Fukushima Medical University School of Medicine (Life Sciences and Social Medicine) Department of Cellular and Integrative Physiology Postdoctoral Researcher
2019.4 - Now
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Fukushima Medical University School of Medicine (Human and Natural Sciences) Department of Natural Sciences (The Section of Advanced Chemistry) Professor
2017.2 - 2019.3
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Professor
2010.4 - Now
Professional Memberships
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Japanese Electrophoresis Society
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The Division of Electrophoresis, The Japan Society for Analytical Chemistry
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その他
Committee Memberships
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日本電気泳動学会 理事
2018.4 - 2020.3
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The Division of Electrophoresis, The Japan Society for Analytical Chemistry The member of standing committee
2018.3 - 2020.2
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Editorial Board of the journal "Electrophoresis (Wiley-VCH, Weinheim)"
Papers
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Shimura Kiyohito
Electrophoresis Letters 62 ( 1 ) 19 - 21 2018.7（ ISSN:2189-2628 ）
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バイオ医薬÷電気泳動:電気泳動でみるバイオ医薬品の特性 免疫アフィニティー捕捉とキャピラリー等電点電気泳動を一体化した分析法のタンパク質医薬分析における可能性
志村 清仁
電気泳動 62 ( 1 ) 19 - 21 2018.7（ ISSN:2189-2628 ）
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Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism
Kobayashi Daisuke, Kakinouchi Kei, Nagae Tomoki, Nagai Toshihiko, Shimura Kiyohito, Hazama Akihiro
FEBS Letters 591 ( 5 ) 718 - 727 2017.2
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Capillary isoelectric focusing after sample enrichment with immunoaffinity chromatography in a single capillary
Kiyohito Shimura and Toshihiko Nagai
Sci. Rep. 6 39221 2016.12（ ISSN:2045-2322 ）
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Direct coupling of immobilized metal ion affinity chromatography and capillary isoelectric focusing in a single capillary
Kiyohito Shimura and Toshihiko Nagai
J. Electrophoresis 59 ( 1 ) 7 - 17 2015.12
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Pattern analysis of post-translational modification using APCE
Kiyohito Shimura
Electrophoresis Letters 58 ( 2 ) 74 - 76 2014.10（ ISSN:0031-9082 ）
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【最新の電気泳動技術】 アフィニティープローブキャピラリー電気泳動による翻訳後修飾パターン分析
志村 清仁
生物物理化学 58 ( 2 ) 74 - 76 2014.10（ ISSN:0031-9082 ）
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Affinity probe capillary electrophoresis of insulin using a fluorescence-labeled recombinant Fab as an affinity probe
Kiyohito Shimura, Ken-ichi Kasai
Electrophoresis 35 ( 6 ) 840 - 845 2014.3
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Estimation of the deamidation rates of major deamidation sites in a Fab fragment of mouse IgG1-kappa by capillary isoelectric focusing of mutated Fab fragments
Shimura, Kiyohito; Hoshino, Makoto; Kamiya, Kei-ichiro; Enomoto, Manabu; Hisada, Sunao; Matsumoto, Hiroyuki; Novotny, Mark; Kasai, Ken-ichi
Anal. Chem. 85 ( 3 ) 1705 - 10 2013.1
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Quantitative evaluation of lectin-reactive glycoforms of alpha(1) -acid glycoprotein using lectin affinity capillary electrophoresis with fluorescence detection
Shimura, K., Tamura, M., Toda, T., Yazawa, S., Kasai, K.
Electrophoresis 32 ( 16 ) 2188 - 2193 2011.6（ ISSN:0173-0835 ）
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Two-step perpendicular free-solution isoelectric focusing in a microchamber array chip
Ishibashi, R., Kitamori, T. and Shimura, K.
Lab Chip 10 ( 19 ) 2628 - 2631 2010.10
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Charge heterogeneity of a therapeutic monoclonal antibody conjugated with a cytotoxic antitumor antibiotic, calicheamicin
Maeda, E., Urakami, K., Shimura, K., Kinoshita, M., Kakehi, K.
J. Chromatogr. A 1217 ( 45 ) 7164 - 7171 2010.9
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Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device
Ihara, M., Yoshikawa, A., Wu, Y., Takahashi, H., Mawatari, K., Shimura, K., Sato, K., Kitamori, T. and Ueda, H.
Lab. Chip 10 ( 1 ) 92 - 100 2010.1
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Recent advances in isoelectric focusing in capillary tubes and microchips
Shimura, K.
Electrophoresis 30 ( 1 ) 11 - 28 2009.1（ ISSN:1522-2683 ）
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A capillary holder for scanning detection of capillary isoelectric focusing with laser-induced fluorescence
Takahashi, K., Maruo, Y., Kitamori, T. and Shimura, K.
J. Sep. Sci. 32 ( 3 ) 394 - 398 2009.1（ ISSN:1615-9314 ）
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Isoelectric Focusing in a Microfluidically Defined Electrophoresis Channel
Shimura, K., Takahashi, K., Koyama, Y., Sato, K. and Kitamori, T
Anal. Chem. 80 ( 10 ) 3818 - 3823 2008.5
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Pesticide Analysis by MEKC on a Micro¬chip with Hydrodynamic Injection from Or¬ganic Extract
Smirnova, A., Shimura, K., Hibara, A., Proskurnin, M. and Kitamori, T.
J. Sep. Sci. 31 ( 5 ) 904 - 908 2008.3
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Focusing Separation Methods
Shimura, K.
Bunseki 12 659 - 660 2007.12
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On-chip Connector Valve for Immunoaffinity Chromatography in a Microfluidic Chip
Shimura, K., Koyama, Y., Sato, K. and Kitamori, T
J. Sep. Sci. 30 ( 10 ) 1477 - 1481 2007.7
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Highly Sensitive Detection of Non-Labeled Peptides Using UV Excitation Thermal Lens Microscope/Liquid Chromatography
Hiki, S., Tokeshi, M., Kakuta, M., Mawatari, K., Kikutani, Y., Sato, K., Hibara, A., Shimura, K., Uchida, N. and Kitamori, T.
Bunseki Kagaku 56 ( 1 ) 1 - 7 2007.1
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Application of a Micro Multiphase Laminar Flow on a Microchip for Extraction and De¬termination of Derivatized Carbamate Pesticides
Smirnova, A., Shimura, K., Hibara, A., Proskurnin, M. and Kitamori, T.
Anal. Sci. 23 ( 1 ) 103 - 107 2007.1
Books
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Capillary Electromigration Separation Methods
Colin F. Poole（Capillary Isoelectric Focusing）
Elsevier 2018.4 （ ISBN:9780128093757 ）
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Recent development of capillary electrophoresis and microchip electrophoresis
Shimura, K.
Japanese Electrophoresis Society 2008.9
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Uh-huh! Q & A's in Protein Research
Toda, T., Hirano, H., Nakamura, K.（What is "affinophoresis"?）
Yodosya 2005.10 （ ISBN:9784897064888 ）
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The front edge of analytical methods, from scientific engineering to nano-bio
Umezawa, Y., Sawada, T., Terabe, S.（Gel electrophoresis）
NTS 2004.12 （ ISBN:4-86043-067-0 ）
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Principles of Biochemistry (Horton, Moran, Ochs, Rawn, Scrimgeour), Third Edition
（Chapter 11-14）
Tokyo Kagaku Dojin 2003.9 （ ISBN:4-8079-0575-9 ）
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Recognition of Carbohydrates in Biological Systems, Part A, General Procedures, Methods in Enzymology, vol. 362
Lee, R. T., Lee, Y. C.（Analysis of Lectin-Carbohydrate Interactions by Capillary Affinophoresis）
Academic Press 2003 （ ISBN:0-12-182265-6 ）
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Saishin Denkieidou Jikkennhou (Principles and Applications of Electrophoresis
Japanese Electrophoresis Society（Affinophoresis）
Ishiyaku Publishers Inc. 1999.2 （ ISBN:4-263-22230-X ）
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Analytical and Preparative Separation Methods of Biomacromolecules
Aboul-Enein, H.Y.（Determination of Affinity Constant of Lectins for Sugars by Affinity Capillary Electrophoresis）
Marcel Dekker, Inc. 1999 （ ISBN:0-8247-1996-4 ）
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Principles of Biochemistry (Horton, Moran, Ochs, Rawn, Scrimgeour), Second Edition
（Chapter 12-15）
Tokyo Kagaku Dojin 1998.4 （ ISBN:4-8079-0478-7 ）
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Quantitative Analysis of Biospecific Interactions
Lundahl, P., Lundqvist, A. and Greijer, E.（Determination of Affinity Constants by Capillary Electrophoresis）
Harwood Academic Publishers 1998 （ ISBN:90-5702-378-4 ）
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High Resolution Separation and Analysis of Biological Macromolecules, Rart B, Ap-plications, Methods in Enzymology, vol. 271
Karger, B. L. and Hancock, W. S.（Affinophoresis: Selective Electrophoretic Separation of Proteins by using a Specific Carrier）
Academic Press 1996 （ ISBN:0-12-182172-2 ）
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Methods of Cell Separation (P. T. Sharpe)
Tokyo Kagaku Dojin 1991.11 （ ISBN:4-8079-1152-X ）
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The New Experimental Course for Biochemistry, Vol. 1, "Protein I"
Japanese Biochemical Society（Chapter 4, Detection of Protein; Chapter 5, Determination of Protein）
Tokyo Kagaku Dojin 1990.2 （ ISBN:4-8079-1047-7 ）
Presentations
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Capillary isoelectric focusing coupled with immunoaffinity: Two micro platforms for analysis of charge variants produced by post translational modifications
MSB 2018: The 34th International Symposium on Microscale Separation and Bioanalysis
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Immunoelectrophoresis instrument for new bio-markers
New Technology Presenting Meetings
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The issues and solutions in the analysis of charge variants of erythropoietin by APCIEF
The 37th Symposium on Capillary Electrophoresis
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Optimization of isoelectric point-variants analysis of erythropoietin by APCIEF
The 68th general meeting of Japanese Electrophoresis Society
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Investigation of IEF conditions with ultra violet absorption detection using PDMA-coated capillaries
The 68th general meeting of Japanese Electrophoresis Society
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Direct coupling of immune extraction and capillary isoelectric focusing in analysis of protein drugs
The 66th Annual Meeting of the Japan Society for Analytical Chemistry
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The prospect of direct coupling of immune extraction and capillary isoelectric focusing in analysis of protein drugs
The 67th Symposium of Japanese Electrophoresis Society: Characterization of Biologics by Electrophoresis
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Direct coupling of immunomagnetic separation and capillary isoelectric focusing
The 36th Symposium on Capillary Electrophoresis (SCE 2016)
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Analysis of isoelectric pattern of erythropoietin using a unified capillary device
The 67th General Meeting of Japanese Electrophoresis Society
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The 67th General Meeting of Japanese Electrophoresis Society
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BIT's 8th Annual International Congress of Antibodies-2016
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32nd International Symposium on Microscale Separation and Bioanalysis (MSB 2016)
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Determination of a pI pattern of recombinant erythropoietin with APCE
The 35th Symposium on Capillary Electrophoresis (SCE 2015)
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The 66th General Meeting of the Japanese Electrophoresis Society
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Electrophoresis Instrument for Direct Coupling of Affinity Chromatography and Capillary Isoelectric Focusing
The 34th Symposium on Capillary Electrophoresis (SCE 2014)
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On-line Coupling of Immunoaffinity Chromatography and CIEF in a Single Capillary
14th Asia-Pacific International Symposium on Microscale Separations and Analysis
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On-line coupling of Anti-E-tag antibody-immobilized column and capillary isoelectric focusing
The 65th Annual Meeting of Japanese Electrophoresis Society
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Pattern analysis of post-translational modifications using APCE
The 65th Annual Meeting of Japanese Electrophoresis Society, Symposium 2 "The current electrophoresis technologies"
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金属キレートクロマトグラフィーとキャピラリー等電点電気泳動のオンライン結合
第74回分析化学討論会
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DIRECT COUPLING OF AFFINITY CHROMATOGRAPHY AND CAPILLARY ISOELECTRIC FOCUSING IN A SINGLE CAPILLARY
30th International Sumposium on MicroScale Bioseparations (MSB 2014)
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翻訳後修飾パターン解析に向けたアフィニティープローブCE (APCE) の開発
「分離機能とセンシング機能の化学」セミナー 2014
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Affinity chromatography of His-tagged protein in a capillary
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Capillary electrophoresis - To achieve real high-sensitivity and removal of interfering components -
The 64th General Meeting of Japanese Electrophoresis Society
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Sample pre-treatment for capillary isoelectric focusing by using capillary inner wall as a solid-phase support
The 33rd Symposium on Capillary Electrophoresis
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キャピラリー電気泳動技術 ? 実質感度の向上と妨害成分の除去を目指して ?
第64回 日本電気泳動学会総会
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APCE for the Analysis of Protein Isoforms in Biological Fluids
SCE 2012, The 32nd Symposium on Capillary Electrophoresis
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Inner wall-coated capillary for electrophoretic analysis of ions in cells, The 2nd report
The 63rd General Meeting of the Japanese Electrophoresis Society
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Recent Advance in APCE
The 62th Symposium of Japanese Electrophoresis Society
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Formation of soluble fragments of an insoluble protein and their detection by APCE
The 62nd Annual General Meeting of the Japanese Electrophoresis Society
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Inner wall coated capillary for electrophoretic analysis of ions in cells
The 62nd Annual General Meeting of the Japanese Electrophoresis Society
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APCE of soluble peptides derived from a protein of low solubility
The 31st Symposium on Capillary Electrophoresis
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For the new developments of capillary electrophoresis
The 61th annual meeting of Japanese Electrophoresis Society
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APCE of Insulin using a Recombinant Antibody
29th Symposium on Capillary Electrophoresis
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Affinity Probe Capillary Electrophoresis of Insulin using a Recombinant Affinity Probe
60th General Meeting of the Japanese Electrophoresis Society
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Development of Electrophoresis ~Coming Across with Nano Technologies~
59th Symposium of the Japanese Electrophoresis Society
Industrial property rights
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分離分析用キャピラリーデバイス、分離分析用マイクロ流体チップ、タンパク質又はペプチド分析方法、電気泳動装置、及び分離分析用マイクロ流体チップ電気泳動装置
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CAPILLARY DEVICE FOR SEPARATION AND ANALYSIS, MICROFLUIDIC CHIP FOR SEPARATION AND ANALYSIS, ANALYSIS METHOD FOR PROTEINS OR PEPTIDES, ELECTROPHORESIS INSTRUMENT, AND MICROFLUIDIC CHIP ELECTROPHORESIS INSTRUMENT FOR SEPARATION AND ANALYSIS
Awards
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International-Activity Promotion Award
2009.9
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Kodama Award (Japanese Electrophoresis Society)
1997.10
Research Projects
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電気泳動による新しい免疫応答マーカーの開発（T細胞受容体クローナリティアッセイ）
2017.4 - 2019.3
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Mod-form pattern analysis by on-line coupling of immunoextraction and capillary isoelectric focusing
2014.4 - 2017.3
Kiyohito Shimura, Toshihiko Nagai
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Development of APCE method for the analysis of post-administration isoforms of protein drugs in administered patients
2013.4 - 2014.3
Shimura, Kiyohito, Nagai, Toshihiko
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Development of a rapid, precise and easy test for EPO doping
2011.12 - 2012.7
SHIMURA, Kiyohito, NAGAI, Toshihiko